In Vivo Scientist

Cambridge
11 months ago
Applications closed

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Agenda are looking for a driven in vivo Scientist to spearhead the creation of advanced transgenic avian models for biologic discovery in one of our facilities in Cambridge. This role entails hands-on work with live animals, focusing on the derivation, cultivation, manipulation, and microinjection of primordial germ cells (PGCs), alongside the care and management of transgenic birds. The ideal candidate will collaborate closely with a multidisciplinary team of molecular biologists and discovery specialists, leveraging their expertise to drive forward this impactful project in collaboration with a prominent pharmaceutical company.
Responsibilities

  • Isolate, culture, and manipulate primordial germ cells (PGC) for genetic modifications.
  • Transfect PGCs with targeting vectors and DNA editing reagents.
  • Isolate monoclonal cell lines and ensure their characterization for downstream applications.
  • Perform microinjections to introduce modified PGCs into recipient embryos with precision.
  • Collaborate on genetic modification strategies like CRISPR/Cas9 and homologous recombination.
  • Manage transgenic colonies and implement breeding strategies for maintenance.
  • Work with cross-functional teams to design tailored transgenic strategies for biologic discovery.
  • Ensure compliance with UK government regulations for animal welfare and biosafety, including obtaining necessary permits for research and transport.
    Qualifications
  • A Ph.D. or Master’s degree in Genetics, Molecular Biology, Developmental Biology, or a related field.
  • Practical experience with genome-editing techniques, particularly in avian or other non-mammalian species.
  • Proficiency in working with PGCs, including their derivation, cultivation, manipulation, transfection, and the isolation of monoclonal cell lines.
  • Solid knowledge of molecular biology techniques, such as PCR, QPCR, cloning, and sequencing.
  • Holder of a Home Office Personal Licence, preferably a PIL A for Avian species.
  • Prior experience in generating and characterizing transgenic animal models is highly desirable.
  • Strong ability to critically analyse and effectively communicate in vivo scientific data.
  • Excellent communication and teamwork skills.
    Agenda and its clients only recruit people who are passionate about animal care and welfare, people with integrity who are honest and trustworthy and who have a great work ethic. To enable us to hire the very best people, we will conduct a comprehensive background and pre-employment screening, an essential part of Agenda's recruitment process

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