Proteomics Scientist

Job Description

Main Purpose of the Job

The Proteomics Scientist will work within the Proteomics Platform at JIC to provide proteomics support to a diverse array of researchers. The role will span multiple aspects of complex proteomics experiments, including advising on experimental design, instrument maintenance and operation, method optimisation, data analysis pipelines, interactions with users/clients, training, sample preparation, data processing, presentation of data, and contributions to associated manuscripts.

Key Relationships

Internal:Line manager, proteomics platform manager and, as necessary, other researchers, research support staff and students across the Institute.

External:TSL proteomics team associated with equipment sharing; other clients and collaborators from external institutions; engineers and support specialists associated with proteomics equipment

Main Activities & Responsibilities

PercentageOperate and maintain several advanced mass spectrometers including the requisite software and nano-scale liquid chromatography systems20Prepare samples for proteomics analysis including protein quantification, enzymatic digestion, isobaric labelling, sample clean up20Run samples on a nanoLCMS system with an Orbitrap Eclipse mass spec for proteomics analysis20Process mass spec raw data for peptide/protein identification and quantification10Support proteomics projects through employing efficient and accurate data evaluation techniques, placing levels of statistical confidence on data output, and clearly reporting results10Interact with faculty and staff and present technical data through presentations and report writing5Train others on procedures, operations and new technology, or methods5Ensure research and record keeping is carried out in accordance with good practice, Scientific Integrity and in compliance with local policies and any legal requirements5Contribute methods, data and analysis to associated research publications5As agreed with line manager, any other duties commensurate with the nature of the role

Person Profile

Education & Qualifications

RequirementImportancePhD or other research role with significant proteomics experienceEssentialMSc/BSc or equivalent in biological sciencesEssential

Specialist Knowledge & Skills

RequirementImportanceExpertise in running and analysing proteomics experimentsEssentialProficiency in quantative proteomics methods, such as isobaric labelling mEssentialProficiency with common mass spectrometry proteomics data analysis softwareEssentialKnowledge in protein separation and enrichment sciences.EssentialKnowledge and interest in biological protein structure, function and interaction.EssentialProficiency in data dependent and data independent acquisition modes Desirable

Relevant Experience

RequirementImportanceExperience of oral research presentations and written presentationsEssentialExperience of supervising, training and mentoring visitors and studentsDesirablePreparing data / information for grant proposalsDesirableExperience in working with different types of mass spectrometers including Orbitrap and MALDI-TOF DesirableExperience with mass spectrometry imagingDesirable

Interpersonal & Communication Skills

RequirementImportanceExcellent communication skills, both written and oral, including the ability to present complex information with clarity EssentialGood interpersonal skills, with the ability to work as part of a teamEssentialDemonstrated ability to work independently, using initiative and applying problem solving skillsEssentialExcellent time management and organisational skillsEssentialAbility to collaborate with internal and external stakeholdersEssentialAbility to follow instructions/Standard Operating ProceduresEssentialPromotes and strives for continuous improvementEssential

Additional Requirements

RequirementImportancePromotes equality and values diversityEssentialWillingness to embrace the expected values and behaviours of all staff at the Institute, ensuring it is a great place to workEssentialAble to present a positive image of self and the Institute, promoting both the international reputation and public engagement aims of the Institute EssentialAbility to maintain confidentiality and security of information where appropriate EssentialWillingness to work outside standard working hours when required EssentialAbility to undertake occasional travel (national and international) related to collaborations and/or seminars EssentialAttention to detailEssential

Who We Are

John Innes Centre

The John Innes Centre is an independent, international centre of excellence in plant and microbial genetics, carrying out fundamental and strategic research, to train scientists and making our findings available to society.

Our research makes use of a wide range of disciplines in the biological and chemical sciences including; cell biology, biochemistry, chemistry, genetics and molecular biology.

For more information about working at the John Innes Centre, please click here.

We are proud to hold a prestigious Athena SWAN Gold award, recognising the impact of our work in promoting gender equality in science. We are also a proud member of the Stonewall Diversity Champion’s programme, a founding signatory of the Tecnician Commitment and, as a Disability Confident employer, guarantee to offer an interview to all disabled applicants who meet the essential criteria for the post.

The John Innes Centre is a registered charity (No. 223852), limited by guarantee (registered in England No. 511709).

Department

Biochemistry and Metabolism

Researchers within the Department of Biochemistry and Metabolism investigate the structure and function of biological macromolecules and fundamental aspects of plant and microbial metabolism. They aim to understand the mechanisms underpinning metabolic diversity and how to manipulate proteins, carbohydrates, small molecules and DNA, research which is facilitating advances of agricultural, environmental, medical and industrial importance.

Group Details

The position will be placed in the JIC Proteomics Facility team and help provide a comprehensive Proteomics service to JIC, as well as for the wider Norwich Research Park and other external partners.

Living in Norfolk