Proteomics Scientist

The John Innes Centre (JIC) seeks a talented, motivated individual as a proteomics specialist to serve the growing needs of proteomics-based technology at JIC, which is a globally recognized, independent research institute specializing in plant and microbial sciences.

About the Proteomics Platform Group

The position will be in the JIC Proteomics Platform team, which provides a comprehensive Proteomics service to JIC, as well as for the wider Norwich Research Park and other external partners. The platform is equipped with state-of-the-art mass spectrometers, including a Thermo Orbitrap Eclipse Tribrid equipped with a FAIMS PRO DUO interface. A MALDI source is also available to perform MALDI imaging on the Orbitrap Eclipse.

Further instruments include a Bruker MALDI-TOF and a Waters Synapt G2-Si Q-TOF. These facilities allow for a broad range of methods including analysis of individual proteins, the qualitative and quantitative analysis of complex protein samples (e.g. CoIPs, expression studies, modifications, isotope labelling), as well as mass spectrometry imaging and ion mobility. We offer collaboration, service and advice across the Norwich Research Park, the wider research community and commercial users.

The role

Applications are invited for an enthusiastic and talented proteomics specialist to join our Proteomics Platform in a full-time, indefinite post. The specialist will work alongside an experienced platform manager to support proteomics projects, including instrument maintenance and operation, sample preparation, method optimization, data analysis pipelines, interactions with users/clients, advising on experimental design, and training. The specialist will also assist with data processing and contribute to associated research papers.

While the work is focused on proteomics on the Orbitrap Eclipse, the role will also involve the operation and maintenance of several advanced mass spectrometers (Thermo Orbitrap Eclipse, Bruker MALDI-TOF, Waters Synapt Q-TOF) including the requisite software and nano-scale liquid chromatography systems. The specialist will prepare samples for proteomics analysis including protein quantification, enzymatic digestion, isobaric labelling, and sample clean up. Projects will be further supported by employing efficient and accurate data evaluation techniques, and the role will involve close interactions with research groups to aid with experimental design, data analysis and associated training.

The ideal candidate

The ideal candidate will have substantial experience in proteomics-based work and a PhD in a relevant subject, or equivalent research experience in proteomics. Hands-on experience in the application of mass spectrometry/LC-MS techniques to the detailed characterization of proteins is essential for this role. Prior experience of running quantitative MS experiments is essential.

The candidate will have knowledge in protein separation and enrichment sciences, as well as an interest in biological protein structure, function and interactions. Knowledge and proficiency in the operation of advanced Orbitrap, MALDI and Q-TOF mass spectrometers is highly desirable, along with associated liquid chromatography systems.

The candidate should be skilled in the preparation of high-quality samples for advanced experiments, such as protein quantification and isobaric labelling. Proficiency with common mass spectrometry proteomics data analysis software is desirable (e.g. Mascot, Proteome Discoverer, MaxQuant, MSFragger, DIA-NN, Skyline), along with experience in handling large data sets and performing statistical data analysis of proteomics results. Experience in working with different types of mass spectrometers, such as mass spec imaging (MALDI-imaging), is desirable.

While experience in instrument maintenance is highly desirable, the platform will provide full training across all relevant instruments and techniques, ensuring you’ll be equipped to handle a variety of tasks.